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RB@LCP-AR diminishes neuroinflammation and restores neuronal synapses in AD mice. AD mice were intravenously injected with the indicated formulations 5 times, then the brain tissues were collected for further analysis. (A–B) The levels of inflammatory TNF-α (A) and IL-6 (B). (C–D) The levels of MDA (C) and SOD (D). (E–G) The distribution and quantitative analysis of Iba-1 (astrocytes) and GFAP (microglia) in the brain tissues. Scale bar = 400 μm. (H–J) The distribution and quantitative analysis of <t>PSD95</t> (postsynaptic) and synaptophysin (presynaptic) in the brain tissues. Scale bar = 400 μm. Data are presented by mean ± SEM (n = 6). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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RB@LCP-AR diminishes neuroinflammation and restores neuronal synapses in AD mice. AD mice were intravenously injected with the indicated formulations 5 times, then the brain tissues were collected for further analysis. (A–B) The levels of inflammatory TNF-α (A) and IL-6 (B). (C–D) The levels of MDA (C) and SOD (D). (E–G) The distribution and quantitative analysis of Iba-1 (astrocytes) and GFAP (microglia) in the brain tissues. Scale bar = 400 μm. (H–J) The distribution and quantitative analysis of PSD95 (postsynaptic) and synaptophysin (presynaptic) in the brain tissues. Scale bar = 400 μm. Data are presented by mean ± SEM (n = 6). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Journal: Materials Today Bio

Article Title: An anti-inflammatory neuroenhancer mitigates amyloid-β pathology to improve Alzheimer's disease therapy

doi: 10.1016/j.mtbio.2026.102874

Figure Lengend Snippet: RB@LCP-AR diminishes neuroinflammation and restores neuronal synapses in AD mice. AD mice were intravenously injected with the indicated formulations 5 times, then the brain tissues were collected for further analysis. (A–B) The levels of inflammatory TNF-α (A) and IL-6 (B). (C–D) The levels of MDA (C) and SOD (D). (E–G) The distribution and quantitative analysis of Iba-1 (astrocytes) and GFAP (microglia) in the brain tissues. Scale bar = 400 μm. (H–J) The distribution and quantitative analysis of PSD95 (postsynaptic) and synaptophysin (presynaptic) in the brain tissues. Scale bar = 400 μm. Data are presented by mean ± SEM (n = 6). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Next, the sections were incubated with HRP-conjugated goat anti-mouse/rabbit IgG polymer for 20 min. To detect the synaptic protein, the sections were incubated with the primary antibody against synaptophysin and PSD95, and then incubated with corresponding secondary antibodies conjugated to Alexa Fluor 488 (Santa Cruz Biotechnology, USA) or Alexa Fluor 594 (Santa Cruz Biotechnology, USA), respectively.

Techniques: Injection